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next-generation sequencing data analysis workflow  (Thermo Fisher)


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    Thermo Fisher next-generation sequencing data analysis workflow
    Next Generation Sequencing Data Analysis Workflow, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+sequencing+data+analysis/Thermo+Sequenase+Cycle+Sequencing+Kit/pm37224176-79-15-20
    Average 90 stars, based on 1 article reviews
    next-generation sequencing data analysis workflow - by Bioz Stars, 2026-10
    90/100 stars

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    Software:

    Article Title: Applications of massively parallel sequencing in forensic genetics
    Article Snippet: .. All these kits were optimized for use in degraded DNA samples and the raw data can be analyzed with Thermo Fisher’s Converge Software NGS Data Analysis module. .. Another product line manufactured by Applied Biosystems, the Ion AmpliSeq Community Panels for Human Identification, is also compatible with the Precision ID sequencing workflow and comprises five solutions.

    Article Title: Applications of massively parallel sequencing in forensic genetics
    Article Snippet: .. All these kits were optimized for use in degraded DNA samples and the raw data can be Figure 1 – Major advancements in forensic genetics related to MPS use. analyzed with Thermo Fisher’s Converge Software NGS Data Analysis module. .. Another product line manufactured by Applied Biosystems, the Ion AmpliSeq Community Panels for Human Identification, is also compatible with the Precision ID sequencing workflow and comprises five solutions.

    Article Title: A multiparametric extraction method for Vn96-isolated plasma extracellular vesicles and cell-free DNA that enables multi-omic profiling
    Article Snippet: ISPs were then loaded onto Ion PI chip and sequenced using Ion Torrent Proton Sequencer. .. Raw reads from sRNAseq were processed with the Torrent Suite Software (v5.4.0, https://www.thermofisher.com/ca/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-data-analysis-workflow/ion-torrent-suite-software.html , accessed on 28/01/2021), which runs the Torrent Mapping Alignment Program (TMAP) using the human reference genome version GRCh37/hg19. ..

    Next-Generation Sequencing:

    Article Title: Applications of massively parallel sequencing in forensic genetics
    Article Snippet: .. All these kits were optimized for use in degraded DNA samples and the raw data can be analyzed with Thermo Fisher’s Converge Software NGS Data Analysis module. .. Another product line manufactured by Applied Biosystems, the Ion AmpliSeq Community Panels for Human Identification, is also compatible with the Precision ID sequencing workflow and comprises five solutions.

    Article Title: Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan.
    Article Snippet: .. We thank Ryoka Miki (Thermo Fisher Scientific) for data analysis of next- generation sequencing, and Kaoru Makabe, Akiko Kasagawa, Akira Kukubun, Akiko Suganuma and Kaoru Watanabe (Equine Research Institute, Japan Racing Association) for their invaluable technical assistance. ..

    Article Title: Applications of massively parallel sequencing in forensic genetics
    Article Snippet: .. All these kits were optimized for use in degraded DNA samples and the raw data can be Figure 1 – Major advancements in forensic genetics related to MPS use. analyzed with Thermo Fisher’s Converge Software NGS Data Analysis module. .. Another product line manufactured by Applied Biosystems, the Ion AmpliSeq Community Panels for Human Identification, is also compatible with the Precision ID sequencing workflow and comprises five solutions.

    other:

    Article Title: Changes in the gut microbiota of NOD mice in response to an oral Salmonella-based vaccine against type 1 diabetes.
    Article Snippet: The amplicon library was cleaned and barcoded, followed by emulsion PCR using the Ion Torrent next-generation sequencing data analysis workflow (Thermo Fisher Scientific).

    Article Title: Added Value of 50-Gene Panel Sequencing to Distinguish Multiple Primary Lung Cancers from Pulmonary Metastases: A Systematic Investigation.
    Article Snippet: Next-generation sequencing and data analysis The Ion ChefTM System and Ion PGMTM IC200 Kit (Life Technologies) were used for preparation of the Ion Torrent sequencing chips (Ion 316 or 318TMV2 chips, Life Technologies) followed by sequencing using the Ion Torrent Personal Genome Machine® (PGM) System, all according to the manufacturer’s protocol.

    Article Title: Control of Long-Term Synaptic Potentiation and Learning by Alternative Splicing of the NMDA Receptor Subunit GluN1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CNQX disodium salt Tocris Cat. No. 1045 (-)-Bicuculline methochloride Tocris Cat. No. 0131 D-AP5 Tocris Cat. No. 0106 Neurobasal medium Thermo Fisher Scientific Cat. No. 21103049 BDNF Peprotech Cat. No. 450-02 GDNF Peprotech Cat. No. 450-10 Cyclic AMP Sigma Cat. No. D0260 Ascorbic acid Sigma Cat. No. A4403 Laminin Roche Cat. No. 11243217001 B27 GIBCO Cat. No. 12587-010 N2 GIBCO Cat. No. 17502-048 Penicillin/streptomycin Invitrogen Cat. No. 15140-122 GlutaMAX Invitrogen Cat. No. 35050061 Spermine tetrahydrochloride Tocris Cat. No. 0958 Glycine Sigma-Aldrich Cat. No. G5417 Magnesium chloride hexahydrate Sigma-Aldrich Cat. No. M2670 N-Methyl-D-aspartic acid Sigma-Aldrich Cat No. M3262 Zinc chloride Sigma-Aldrich Cat No. 229997 RNAlater Stabilization Solution Thermo Fisher Scientific Cat No. AM7021 Critical Commercial Assays BrainStain Imaging Kit Thermo Fisher Scientific Cat# B34650 KAPA Long Range HotStart PCR Kit KAPA Biosystems Cat No. KK3501 QIAShredder QIAGEN Cat No. 79656 RNeasy Lipid Tissue Mini Kit QIAGEN Cat No. 74804 Maxima H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat No. K1652 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat No. 4444965 Deposited Data Next Generation RNA Sequencing data from brain https://www.ncbi.nlm.nih.gov/ gds GEO: GSE136983 Experimental Models: Cell Lines Control and proband 1 iPSCs Ross et al., 2019 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6N-Tyr_c N4 Charles River Labs Crl#493 Mouse: B6.FVB-Tg(EIIa-cre)C5379Lmgd/J The Jackson Laboratory Jax 003724 Mouse: 129S4/SvJaeSor-Gt(ROSA) 26Sortm1(FLP1)Dym/J The Jackson Laboratory Jax 003946 Mouse: GluN1a This paper N/A Mouse: GluN1b This paper N/A Oligonucleotides Gapdh(MM99999915_G1) Thermo Fisher Scientific Cat No. 4331182 Custom Oligonucleotides for PCR and genotyping are reported in Table S1 This paper N/A (Continued on next page) Cell Reports 29, 4285–4294.e1–e5, December 24, 2019 e1

    Article Title: Recurrent genetic alterations and biomarker expression in primary and metastatic squamous cell carcinomas of the vulva
    Article Snippet: The tissue pellet was resuspended in Buffer ATL (Qiagen) with added proteinase K. The rest of the procedure followed the manufacturer’s instruction.



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